BCOUniversity

1. Proteinanalysis

2. Databases

3. 2-D protocols




Protein function

  • Swiss-Prot and TrEMBL
  • NCBI
  • PEDANT (Protein Extraction Description, and Analysis Tool)
  • Protein Database Searching of GenomeWeb (collection of protein database sites)

    • Protein characteristics

    Diverse tools
  • ExPASy Molecular Biology Server
    • Prediction of expected spot position within the 2-D gel (pI, MM)
  • Compute pI/Mw (ExPASy)
    • Prediction of MTS (Mitochondrial Targeting Sequence)
  • TargetP
  • PSORT
  • MitoProt
    • Prediction of transmembrane sequences and GRAVY score
  • SOSUI

    • Protein identification (mass spectrometry)

  • ProFound
  • MS-Fit
  • Mascot


    • Mitochondria

    General databases
  • General information (Cell Biology topics: Mitochondria)
  • Mitochondria Project Database (MITOP2)
  • Mendelian Inheritance and the Mitochondrion (MitoDat)
  • The Mitochondria Research Society
  • Mitochondrial Disorders (Washington University School of Medicine)
  • A human mitochondrial genome database (MITOMAP)
  • Human Mitochondrial Protein Database
  • Complete mitochondrial genome sequences (NCBI)
    • 2-D databases
  • Arabidopsis thaliana mitochondrial proteome (University of Hanover)
  • Human and mouse mitochondrial proteome (Mito 2D)

    • Escherichia coli

    General databases
  • The E. coli index
  • Colibri
    • 2-D Databases
  • Whole cellular extract (SWISS-2DPAGE)

    • Yeast

    General databases
  • The World-Wide Web Virtual Library: Yeast
  • Saccharomyces Genome Database (SGD)
  • Comprehensive Yeast Genome Database (CYGD)
  • Subcellular localization of the yeast proteome (according to Kumar et al., 2002)
  • Subcellular localization of the yeast proteome (according to Huh et al., 2003)
  • Yeast Transport Protein database (YTPdb)
    • 2-D Databases
  • Whole cellular extract (SWISS-2DPAGE)
  • Whole cellular extract (Yeast Protein Map, Bordeaux)
  • Alkaline proteins (2-DE database of S. cerevisiae, Munich)
  • Secreted proteins (COMPLUYEAST-2DPAGE, Madrid)

    • Mouse

    General databases
  • emap (edinburgh mouse atlas project)
  • uMRI Atlas of Mouse Development
    • 2-D Databases
  • see ExPASy for an overview

    • Human

    General databases
  • Human Protein Reference Database
  • HUPO (Human Proteome Organisation)
  • images.MD (online encyclopedia of medical images)
    • 2-D Databases
  • see ExPASy for an overview

    • 2-D databases of other organisms

  • List of 2-D PAGE Databases (ExPASy)
  • Partial List of Web 2D Electrophoretic Gel Databases (LECB)
  • Search for other databases (2DWG Image Meta-Database)
  • Search for other databases (2DHunt)



  • Proteomic Group of Prof. Dr. Angelika Görg (Technical University of Munich)
  • SWISS-2DPAGE protocols



    • Questions? Comments?
    Please contact Steffen Ohlmeier