Separation of low molecular mass proteins dependent on the pH and temperature
1. pH 8.80 at 12 °C		2. pH 8.80 at 15 °C		3. pH 8.80 at 21 °C
4. pH 8.80 at 24 °C		5. pH 8.80 at 27 °C		6. pH 8.80 at 30 °C
7. pH 8.80 at 33 °C
		We commonly use a 1.5 M Tris/ HCl solution calibrated at 27 °C (5.), which allows the detection of proteins down to a molecular mass of approx. 6 kDa.