Influence of the temperature on the pH in the 1.5 M Tris/ HCl solution
Blue: calibrated 1.5 M Tris/ HCl solution (pH 8.80 was calibrated at 27 °C and reflects pH 9.22 at 12 °C)
Red: pH in the complete slab gel solution is pH 9.18 during running conditions at 12 °C