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| 1. Bacteria |
| Escherichia coli | |
| Whole cell extract (pH 4-7, 100 µg)  |
Whole cell extract (pH 3-10NL, 100 µg)  |
| approx. 1200 spots | approx. 1200 spots |
| Preparation protocol | |
| 2. Fungi |
| Saccharomyces cerevisiae | ||
| Whole cell extract (pH 3-10NL, 100 µg)  |
Mitochondria (pH 3-10NL, 100 µg)  |
Mitochondria (pH 3-10NL, 600 µg)  |
| approx. 1400 spots | approx. 1200 spots | approx. 1400 spots |
| Preparation protocol | Preparation protocol | |
| For more information see: | ||
| YMP (Yeast Mitochondrial Proteome 2-D database)
or Ohlmeier et al. (2004) | ||
| 3. Higher eukaryotes |
| Mouse | ||
| Kidney (pH 3-10NL, 100 µg)  |
Bone (calvaria) (pH 3-10NL, 100 µg)  |
Bone (femur) (pH 3-10NL, 100 µg)  |
| approx. 1300 spots | approx. 1200 spots | approx. 1350 spots |
| Preparation protocol | Preparation protocol | |
| T-cells from spleen (pH 3-10NL, 100 µg)  |
Mouse fibroblast cell line NIH3T3, intracellular (pH 3-10NL, 100 µg)  |
| approx. 1300 spots | approx. 1100 spots |
| Preparation protocol | Preparation protocol |
| Human | ||
| Serum (pH 3-10NL, 200 µg)  |
Tooth (pulp tissue) (pH 3-10NL, 500 µg)  | |
| approx. 500 spots | approx. 650 spots | |
| Preparation protocol | Preparation protocol | |
| For more information see: | ||
| Tooth pulp 2-D database | ||
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Questions? Comments? Please contact Steffen Ohlmeier | |
