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2-D reference gelIdentified proteinsComparison


1. Mitochondrial proteome (pH 3-10) specific for fermentation
1.1. Reference 2-D gel: Click on a spot to get more information
1.2. 2-D gel divided into 4 parts: Click on a part to enlarge this region
1.3. 2-D reference map as Melanie-file via Internet Explorer (8.9 MB)

2. Mitochondrial proteome (pH 3-10) specific for respiration
2.1. Reference 2-D gel: Click on a spot to get more information
2.2. 2-D gel: Click on the gel to view selected regions presenting proteins with changed expression
2.3. 2-D reference map as Melanie-file via Internet Explorer (8.5 MB)


1. Mitochondrial proteome (pH 3-10) specific for fermentation


1.1. Reference 2-D gel: Click on a spot to get more information

The mitochondrial proteins (100 µg) from a glucose grown yeast culture (optical density at 600 nm of 1.1) were purified according to Meisinger et al., 2000 separated in a non linear pH gradient 3-10 and stained with silver nitrate.

Identified spots are marked with a hand icon.
  • MouseOver = protein identity
  • MouseClick = detailed protein information


  • 1.2. 2-D gel divided into 4 parts: Click on a part to enlarge this region

    Proteins detected only in the protein pattern specific for respiratory growth were marked with an asterisk. Spots detected in less than 11 of the 12 analyzed extracts were marked with brackets.

    pH 310
    NW NO
    SW SO


    1.3. 2-D reference map as Melanie-file (8.9 MB)

    Download the tiff file here (via Internet Explorer)




    2. Mitochondrial proteome (pH 3-10) specific for respiration


    2.1. Reference 2-D gel: Click on a spot to get more information

    The mitochondrial proteins (100 µg) from a glycerol grown yeast culture were purified according to Meisinger et al., 2000 separated in a non linear pH gradient 3-10 and stained with silver nitrate.

    Identified spots are marked with a hand icon.
  • MouseOver = protein identity
  • MouseClick = detailed protein information


  • 2.2. 2-D gel: Click on the gel to view selected regions presenting proteins with changed expression

    Only changes in protein expression after the diauxic shift are marked.
    pH 310
    wildtype glycerol

    2.3. 2-D reference map as Melanie-file (8.5 MB)

    Download the tiff file here (via Internet Explorer)




    Questions? Comments?
    Please contact Steffen Ohlmeier