Data Collection

For the X-ray diffraction data collection we have the Nonius FR591 rotating anode X-ray generator equipped with a Montel focusing mirror system. The data is collected with a MAR345 image plate area detector (Figure A and C). Data collection is carried out at room temperature or with cryo-cooled (-170 ^oC) crystals, using a Oxford Cryo system. The X-ray diffraction laboratory is also equipped with an X-Ray Eye camera (Photonic Science) for X-ray beam alignment. The image of the X-Ray Eye can be stored on a PC for later analysis of the beam properties.

Diffraction data is also regularly collected at synchrotrons. Occasionally, the data is collected remotely. For these data collection efforts we maintain storage dewars and transport dewars (Figure D). For the freezing of crystals a range of loops are available, including bar-coded loops, which are in particular important for synchrotron visits. The data processing is done routinely with XDS or iMOSFLM.

Figure A. X-ray diffractometer. On the right is the X-ray generator attached to the focusing mirrors. In the middle is the cryosystems cold head attached to the support stand and on the left is the image plate area detector. Figure B. A close up image of the coniometer head, beam stop and the cryosystem cold head. Figure C. A diffraction image of a TIM crystal. Figure D. A transport dewar.

Future developments

Our equipment has performed well, but some devices are now becoming obsolete and subject to failure. In the context of our Biocenter Finland activities we will in 2011 replace the current unit by a modern microfocus rotating anode, equipped with CCD detector and kappa goniometer, which is a major upgrade. We aim to implement this system in such a way that data tracking will be possible, using EDNA/ISPYB technology (for the collected datasets) as well as XtalPIMS technology (for tracking the crystal and crystal handling).

Additional information

There is much more information available for the local users, on the protein crystallography wiki, accessible to local users via the X-ray homepage.

Updated August 19th, 2011